Efficient Agrobacterium-Mediated Transformation of the Commercial Hybrid Poplar Populus Alba × Populus glandulosa Uyeki.
Identifieur interne : 000997 ( Main/Exploration ); précédent : 000996; suivant : 000998Efficient Agrobacterium-Mediated Transformation of the Commercial Hybrid Poplar Populus Alba × Populus glandulosa Uyeki.
Auteurs : Chengwei Song [République populaire de Chine] ; Liang Lu [République populaire de Chine] ; Yayu Guo [République populaire de Chine] ; Huimin Xu [République populaire de Chine] ; Ruili Li [République populaire de Chine]Source :
- International journal of molecular sciences [ 1422-0067 ] ; 2019.
Descripteurs français
- KwdFr :
- Agrobacterium tumefaciens (génétique), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Glucuronidase (génétique), Glucuronidase (métabolisme), Populus (génétique), Protéines végétales (génétique), Protéines végétales (métabolisme), Transformation génétique (MeSH), Végétaux génétiquement modifiés (génétique).
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Glucuronidase, Plant Proteins.
- genetics : Agrobacterium tumefaciens, Plant Leaves, Plants, Genetically Modified, Populus.
- chemical , metabolism : Glucuronidase, Plant Leaves, Plant Proteins.
- Transformation, Genetic.
Abstract
Transgenic technology is a powerful tool for gene functional characterization, and poplar is a model system for genetic transformation of perennial woody plants. However, the poplar genetic transformation system is limited to a number of model genotypes. Herein, we developed a transformation system based on efficient Agrobacterium-mediated transformation for the hybrid poplar Populus Alba × Populus glandulosa Uyeki, which is a fast-growing poplar species that is suitably grown in the northern part of China. Importantly, we optimized many independent factors and showed that the transformation efficiency was improved significantly using juvenile leaf explants. Explants were infected by an Agrobacterium suspension with the OD600 = 0.6 for 15 min and then co-cultured in dark conditions for 3 days. Using the improved transformation system, we obtained the transgenic poplar with overexpression of β-glucuronidase (GUS) via direct organogenesis without callus induction. Furthermore, we analyzed the GUS gene in the transgenic poplars using PCR, qRT-PCR, and GUS staining. These analyses revealed that the GUS gene was efficiently transformed, and it exhibited various expression levels. Taken together, these results represent a simple, fast, and efficient transformation system of hybrid poplar plants. Our findings may facilitate future studies of gene functions in perennial woody plants and tree breeding via transgenic technology assisted design.
DOI: 10.3390/ijms20102594
PubMed: 31137806
PubMed Central: PMC6566960
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<i>Agrobacterium</i>-Mediated Transformation of the Commercial Hybrid Poplar <i>Populus Alba × Populus glandulosa</i> Uyeki.</title><author><name sortKey="Song, Chengwei" sort="Song, Chengwei" uniqKey="Song C" first="Chengwei" last="Song">Chengwei Song</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China. songchengwei@bjfu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. songchengwei@bjfu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire 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Forestry University, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">International journal of molecular sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Glucuronidase (genetics)</term><term>Glucuronidase (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Plants, Genetically Modified (genetics)</term><term>Populus (genetics)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Glucuronidase (génétique)</term><term>Glucuronidase (métabolisme)</term><term>Populus (génétique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucuronidase</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Plant Leaves</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Feuilles de plante</term><term>Glucuronidase</term><term>Populus</term><term>Protéines végétales</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term><term>Plant Leaves</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Feuilles de plante</term><term>Glucuronidase</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Transformation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Transgenic technology is a powerful tool for gene functional characterization, and poplar is a model system for genetic transformation of perennial woody plants. However, the poplar genetic transformation system is limited to a number of model genotypes. Herein, we developed a transformation system based on efficient <i>Agrobacterium</i>-mediated transformation for the hybrid poplar <i>Populus Alba × Populus glandulosa</i> Uyeki, which is a fast-growing poplar species that is suitably grown in the northern part of China. Importantly, we optimized many independent factors and showed that the transformation efficiency was improved significantly using juvenile leaf explants. Explants were infected by an <i>Agrobacterium</i> suspension with the OD<sub>600</sub> = 0.6 for 15 min and then co-cultured in dark conditions for 3 days. Using the improved transformation system, we obtained the transgenic poplar with overexpression of <i>β-glucuronidase</i> (<i>GUS</i>) via direct organogenesis without callus induction. Furthermore, we analyzed the <i>GUS</i> gene in the transgenic poplars using PCR, qRT-PCR, and GUS staining. These analyses revealed that the <i>GUS</i> gene was efficiently transformed, and it exhibited various expression levels. Taken together, these results represent a simple, fast, and efficient transformation system of hybrid poplar plants. Our findings may facilitate future studies of gene functions in perennial woody plants and tree breeding via transgenic technology assisted design.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31137806</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1422-0067</ISSN><JournalIssue CitedMedium="Internet"><Volume>20</Volume><Issue>10</Issue><PubDate><Year>2019</Year><Month>May</Month><Day>27</Day></PubDate></JournalIssue><Title>International journal of molecular sciences</Title><ISOAbbreviation>Int J Mol Sci</ISOAbbreviation></Journal><ArticleTitle>Efficient <i>Agrobacterium</i>-Mediated Transformation of the Commercial Hybrid Poplar <i>Populus Alba × Populus glandulosa</i> Uyeki.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E2594</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms20102594</ELocationID><Abstract><AbstractText>Transgenic technology is a powerful tool for gene functional characterization, and poplar is a model system for genetic transformation of perennial woody plants. However, the poplar genetic transformation system is limited to a number of model genotypes. Herein, we developed a transformation system based on efficient <i>Agrobacterium</i>-mediated transformation for the hybrid poplar <i>Populus Alba × Populus glandulosa</i> Uyeki, which is a fast-growing poplar species that is suitably grown in the northern part of China. Importantly, we optimized many independent factors and showed that the transformation efficiency was improved significantly using juvenile leaf explants. Explants were infected by an <i>Agrobacterium</i> suspension with the OD<sub>600</sub> = 0.6 for 15 min and then co-cultured in dark conditions for 3 days. Using the improved transformation system, we obtained the transgenic poplar with overexpression of <i>β-glucuronidase</i> (<i>GUS</i>) via direct organogenesis without callus induction. Furthermore, we analyzed the <i>GUS</i> gene in the transgenic poplars using PCR, qRT-PCR, and GUS staining. These analyses revealed that the <i>GUS</i> gene was efficiently transformed, and it exhibited various expression levels. Taken together, these results represent a simple, fast, and efficient transformation system of hybrid poplar plants. Our findings may facilitate future studies of gene functions in perennial woody plants and tree breeding via transgenic technology assisted design.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Chengwei</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China. songchengwei@bjfu.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. songchengwei@bjfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Liang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China. luliang@bjfu.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. luliang@bjfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guo</LastName><ForeName>Yayu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China. guoyau1510@bjfu.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. guoyau1510@bjfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Huimin</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Life Sciences, Peking University, Beijing 100871, China. xuhm@pku.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ruili</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China. liruili@bjfu.edu.cn.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China. liruili@bjfu.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>2019ZY29</GrantID><Agency>Fundamental Research Funds for the Central Universities</Agency><Country></Country></Grant><Grant><GrantID>No. 2016YFD0600102</GrantID><Agency>State '13.5' Key Research Program of China</Agency><Country></Country></Grant><Grant><GrantID>31670182</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>31761133009</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>31530084</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>31401149</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>3180030099</GrantID><Agency>National Natural Science Foundation of China</Agency><Country></Country></Grant><Grant><GrantID>2018M631246</GrantID><Agency>China Postdoctoral Science Foundation</Agency><Country></Country></Grant><Grant><GrantID>111 project, B13007</GrantID><Agency>Program of Introducing Talents of Discipline to Universities</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>27</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int J Mol Sci</MedlineTA><NlmUniqueID>101092791</NlmUniqueID><ISSNLinking>1422-0067</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium tumefaciens</Keyword><Keyword MajorTopicYN="N">P. alba × P. glandulosa Uyeki</Keyword><Keyword MajorTopicYN="N">leaf explants</Keyword><Keyword MajorTopicYN="N">regeneration transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>04</Month><Day>14</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>05</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>05</Month><Day>22</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31137806</ArticleId><ArticleId 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Pékin</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Song, Chengwei" sort="Song, Chengwei" uniqKey="Song C" first="Chengwei" last="Song">Chengwei Song</name></noRegion><name sortKey="Guo, Yayu" sort="Guo, Yayu" uniqKey="Guo Y" first="Yayu" last="Guo">Yayu Guo</name><name sortKey="Guo, Yayu" sort="Guo, Yayu" uniqKey="Guo Y" first="Yayu" last="Guo">Yayu Guo</name><name sortKey="Li, Ruili" sort="Li, Ruili" uniqKey="Li R" first="Ruili" last="Li">Ruili Li</name><name sortKey="Li, Ruili" sort="Li, Ruili" uniqKey="Li R" first="Ruili" last="Li">Ruili Li</name><name sortKey="Lu, Liang" sort="Lu, Liang" uniqKey="Lu L" first="Liang" last="Lu">Liang Lu</name><name sortKey="Lu, Liang" sort="Lu, Liang" uniqKey="Lu L" first="Liang" last="Lu">Liang Lu</name><name sortKey="Song, Chengwei" sort="Song, Chengwei" uniqKey="Song C" first="Chengwei" last="Song">Chengwei Song</name><name sortKey="Xu, Huimin" sort="Xu, Huimin" uniqKey="Xu H" first="Huimin" last="Xu">Huimin Xu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= Efficient Agrobacterium-Mediated Transformation of the Commercial Hybrid Poplar Populus Alba × Populus glandulosa Uyeki.
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